4-Hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase

4-Hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase (EC 3.2.1.182, DIMBOAGlc hydrolase, DIMBOA glucosidase) is an enzyme with systematic name (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside beta-D-glucosidase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction

(1) (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose
(2) (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose
4-Hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase
Identifiers
EC no.3.2.1.182
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Search
PMCarticles
PubMedarticles
NCBIproteins

The enzyme from Triticum aestivum (wheat) has a higher affinity for DIMBOA glucoside than DIBOA glucoside.

References
  1. Sue M, Ishihara A, Iwamura H (February 2000). "Purification and characterization of a hydroxamic acid glucoside beta-glucosidase from wheat (Triticum aestivum L.) seedlings". Planta. 210 (3): 432–8. doi:10.1007/s004250050029. PMID 10750901. S2CID 6266316.
  2. Sue M, Ishihara A, Iwamura H (June 2000). "Purification and characterization of a beta-glucosidase from rye (Secale cereale L.) seedlings". Plant Science. 155 (1): 67–74. doi:10.1016/s0168-9452(00)00204-1. PMID 10773341.
  3. Czjzek M, Cicek M, Zamboni V, Bevan DR, Henrissat B, Esen A (December 2000). "The mechanism of substrate (aglycone) specificity in beta -glucosidases is revealed by crystal structures of mutant maize beta -glucosidase-DIMBOA, -DIMBOAGlc, and -dhurrin complexes". Proceedings of the National Academy of Sciences of the United States of America. 97 (25): 13555–60. Bibcode:2000PNAS...9713555C. doi:10.1073/pnas.97.25.13555. PMC 17614. PMID 11106394.
  4. Nikus J, Esen A, Jonsson LM (2003). "Cloning of a plastidic rye (Secale cereale) β-glucosidase cDNA and its expression in Escherichia coli". Physiol. Plant. 118 (3): 337–348. doi:10.1034/j.1399-3054.2003.00118.x.
  5. Sue M, Yamazaki K, Yajima S, Nomura T, Matsukawa T, Iwamura H, Miyamoto T (August 2006). "Molecular and structural characterization of hexameric beta-D-glucosidases in wheat and rye". Plant Physiology. 141 (4): 1237–47. doi:10.1104/pp.106.077693. PMC 1533919. PMID 16751439.
  6. Sue M, Nakamura C, Miyamoto T, Yajima S (February 2011). "Active-site architecture of benzoxazinone-glucoside β-D-glucosidases in Triticeae". Plant Science. 180 (2): 268–75. doi:10.1016/j.plantsci.2010.09.001. PMID 21421370.
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.