Fructan beta-(2,6)-fructosidase
Fructan beta-(2,6)-fructosidase (EC 3.2.1.154, beta-(2-6)-fructan exohydrolase, levanase, 6-FEH, beta-(2,6)-D-fructan fructohydrolase) is an enzyme with systematic name (2->6)-beta-D-fructan fructohydrolase.[1][2][3] This enzyme catalyses the following chemical reaction
- Hydrolysis of terminal, non-reducing (2->6)-linked beta-D-fructofuranose residues in fructans
| Fructan beta-(2,6)-fructosidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.2.1.154 | ||||||||
| CAS no. | 1000597-62-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
The best substrates are the levan-type fructans such as 6-kestotriose [beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl alpha-D-glucopyranoside] and 6,6-kestotetraose [beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl alpha-D-glucopyranoside].
References
- Marx SP, Nösberger J, Frehner M (1997). "Hydrolysis of fructan in grasses: A β-(2-6)-linkage specific fructan-β-fructosidase from stubble of Lolium perenne". New Phytol. 135: 279–290. doi:10.1046/j.1469-8137.1997.00642.x.
- Van den Ende W, De Coninck B, Clerens S, Vergauwen R, Van Laere A (December 2003). "Unexpected presence of fructan 6-exohydrolases (6-FEHs) in non-fructan plants: characterization, cloning, mass mapping and functional analysis of a novel "cell-wall invertase-like" specific 6-FEH from sugar beet (Beta vulgaris L.)". The Plant Journal. 36 (5): 697–710. doi:10.1046/j.1365-313x.2003.01912.x. PMID 14617070.
- Henson CA, Livingston DP (February 1996). "Purification and characterization of an oat fructan exohydrolase that preferentially hydrolyzes beta-2,6-fructans". Plant Physiology. 110 (2): 639–44. doi:10.1104/pp.110.2.639. PMC 157760. PMID 8742337.
External links
- Fructan+beta-(2,6)-fructosidase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
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