Use MemoryStore in express ONLY if you are not creating multiple instances (such as with the cluster module). If you are load balancing across machines, your load balancer will need to use sticky / persistent sessions.
If you meet those requirements, then all you need to do is upon login, once the credentials are validated, set a session variable to indicate logged in, for example:
req.session.loggedIn = true;
If you want to check if a user is logged in, simply check that variable.
if (req.session.loggedIn) {
// user is logged in.
}
else {
// user is not logged in.
}
You mentioned preventing a single user from having sessions more than one session at a time. To achieve that, you may need to store something in a database indicating that the user is logged in. I warn you, this can be dangerous because of stale sessions. For example, what if a user logs in, but never logs out? What if they close their browser window so the session is gone forever?
Express has no concept of an idle session expiration. I have implemented such a thing by storing all sessions in the database along with a last accessed timestamp and then periodically clean up the session data based on the time. But then you need to update your list of who is logged in as well.